Technology

The John Wayne Cancer Institute Sequencing Center is equipped with an Illumina Hiseq 2500 with two run modes allowing for flexibility and scalability to support a broad range of project sizes and designs. Ideal for whole genome or exome with larger sample sizes, the High Output Mode on the HiSeq 2500 can process up 600 Gb of high quality sequence. For smaller scale projects or targeted sequencing applications, the Rapid Mode on the HiSeq 2500 can produce up to 180 Gb of high quality sequencing in as little as two days.

The Sequencing Center is also equipped with an Illumina MiSeq to offer flexibility and fast turnaround time for sequencing small projects, small genomes, or targeted sequencing panels. The MiSeq also offers longer reads lengths (300 bp) and is fully compatible with all libraries that can be sequenced on the Illumina HiSeq.

Our instruments utilize Illumina’s highly accurate, efficient, and scalable sequencing by synthesis technology. Learn more about sequencing by synthesis here.