Sample Requirements

Quality of starting material is a key factor in producing optimal sequencing data and results. The following are general sample requirement guidelines for our library preparation and sequencing services.

General Guidelines

DNA and/or RNA specimens should be suspended in water, low TE, or Qiagen elution buffer and stored in low binding 1.5 ml tubes. The top and side of each tube should be clearly labeled with the sample name, date, institution and/or PI’s initials. The sample submission form along with the appropriate quality analysis results as detailed below are required for sample submission.

DNA Sample Requirements

Please provide sample analysis results measured by Qubit, Agilent 2100 Bioanalyzer, or Agilent 2200 TapeStation in one or multiple forms. NanoDrop quantification alone is not recommended. For genomic DNA please submit a photograph of the sample separated by agarose gel electrophoresis (1-2%) to confirm the integrity and quality of the sample. Quality genomic DNA should appear as a dark, tight, high molecular weight band with no smearing below 20 kb.

Whole Genome Sequencing

Purity: OD260/280 = 1.8-2.0 without degradation and RNA contamination
Concentration: ≥30ng/µl
DNA amount for each library: ≥2.5µg high quality genomic DNA

Whole Exome Sequencing

Purity: OD260/280 = 1.8-2.0 without degradation and RNA contamination
Concentration: ≥30ng/µl
DNA amount for each library: ≥400 ng high quality genomic DNA or ≥2.5 µg FFPE DNA

RNA Sample Requirements

Please provide analysis results of the RNA sample using at least two of the following methods: Qubit, NanoDrop, Agilent 2100 Bioanalyzer, and Agilent 2200 TapeStation. Please purify samples, avoiding contamination by polycarbonate, protein and exonuclease.

mRNA Sequencing

Purity: OD260/280 = 1.8-2.0; OD260/230 >1.8; RIN ≥ 8 for high quality total RNA or %DV200 ≥ 30% for FFPE total RNA
Concentration: ≥20ng/µl
Total RNA amount for each library: ≥500 ng of high quality total RNA or ≥300 ng of FFPE total RNA

All samples must pass our quality control requirements before they can be processed for sequencing. Please contact us for more detailed sample submission requirements specific to your project.